Generation of high-affinity chicken single-chain Fv antibody fragments for measurement of the Pseudonitzschia pungens toxin domoic acid
Article Abstract:
The isolation and in vitro maturation of a chicken single-chain antibody variable region (scFv) directed against the low-molecular-mass (312-Da) algal toxin domoic acid (DA), its convenient production and purification and application in the development of a simple competitive enzyme immunoassay for detection of the toxin in shellfish extracts are described. The study has showed that chickens provide a valuable model system for the simplified, rapid generation of high-affinity recombinant antibody fragments with specificity for small toxin molecules.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Functional complementation of nontoxic mutant binary toxins of Bacillus sphaericus 1593M generated by site-directed mutagenesis
Article Abstract:
Site-directed mutagenesis was applied to examine the mode of action of the binary toxin formed by the combination of two mosquito larvicidal proteins of 51 and 42kDa. On its own, each of these peptides is nontoxic. When synthesized by Bacillus sphaericus 1593M during sporulation, the peptides of 51 and 42 kDa show toxicity. The impact of the substitution of alanine residues for other amino acids found at the N- and C-terminal regions of the binary the binary toxin on biological activity was investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Role of Bacillus thuringiensis toxin domains in toxicity and receptor binding in the diamondback moth
Article Abstract:
The possible role of domain III of the toxic fragment of Bacillus thuringiensis crystal proteins in toxin specificity and in midgut epithelial membrane binding specificity in the diamondback moth was explored using hybrid toxins with domain III substitutions in Cry1C, Cry1E and Cry1Ab. Results showed that domain II and/or domain I, and not domain III, mediates toxin specificity as well as specificity of binding to membrane vesicles in the diamondback moth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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