Genetic and biochemical characterization of the Lactobacillus delbrueckii subsp. lactis bacteriophage LL-H lysin

Article Abstract:

The gene 'mur' encodes the virulent phage enzyme 'Mur' in the LL-H virulent phage of Lactobacillus delbrueckii. The C-terminal domain of Mur does not have catalytic activity. The N-terminal amino acid sequence follows the nucleotide sequence of the mur gene, which was cloned into Escherichia coli. It is a muramidase. The N-terminal part is homologous to many lysozymes. The temperature and pH optima of Mur are 30-40 degrees celsius and 5.0 respectively.

Enzymes, Cloning, Enzyme structure-activity relationships, Amino acid sequence, Amino acid sequencing

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Mechanism of the citrate transporters in carbohydrate and citrate cometabolism in Lactococcus and Leuconostoc species

Article Abstract:

The energetics of citrate metabolism in Lactococcus lactis and Leuconostoc mesenteroides are examined and compared by characterizing the citrate transporter and reverse expression of this transporter between the two species. Results reveal that the citrate transporters of the two species are nearly identical. Further, both forms of citrate transporter generate an electrochemical proton gradient as they metabolize citrate.

Physiological aspects, Carrier proteins, Transport proteins, Biological transport, Carbohydrate metabolism, Cometabolism, Citrates

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Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon aeration: involvment of an NADH oxidase in oxidative stress

Article Abstract:

Lactobacillus delbrueckii subsp. bulgaricus has an NADH:H(sub)2O(sub)2 oxidase that eliminates oxygen in the environment by converting it to hydrogen peroxide. However, the increased hydrogen peroxide causes the bacterium to enter into a stationary phase earlier than usual.

Oxidation, Physiological, Physiological oxidation, Oxidases

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