Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes
Article Abstract:
A nicaraguan field isolate (SfNIC) of spodoptera frugiperda nucleopolyhedrovirus was purified by plague assay on Sf9 cells and nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. It is concluded that the SfNIC population presents high levels of genetic diversity, localized to a 17-kb, region containing pif and pif-2.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Optical brighteners do not influence covert baculovirus infection of Spodoptera frugiperda
Article Abstract:
The influence of the presence of an optical brightener (Tinopal UNPA-GX) on covert infection with Spodoptera frugiperda multiple nucleopolyhedrovirus is examined by reverse transcription PCR of virus gene transcripts. The analysis shows that the detection of the covert infection is dependent on insect life stage and the viral mRNA used for diagnosis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population
Article Abstract:
A Nicaraguan isolate of a nucleopolyhedrovirus (SfNIC) attacks the fall armyworm Spodoptera frugiperda, survives as a mixture of nine genotypes (SfNIC A to I) that all present genomic deletions, except variant B (complete genotype). The result demonstrates the value of retaining genotypic diversity in virus-based bioinsecticides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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