High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe
Article Abstract:
A P1 genomic library of 17-fold coverage and a cosmid library of eight genome equivalents were ordered by hybridizing genetic markers and individual clones from the two libraries. Both were gridded on high density filters and subdivided by yeast artificial chromosome clones covering the entire genome. Further data was provided by the hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids. This approach of using parallel libraries greatly enhances the mapping and resolution of the clone order, although further improvements can be made in speed and accuracy.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation
Article Abstract:
Research has been undertaken into the CAG/polyglutamine-repeat expansion which is responsible for the human neurodegenerative disorder Huntington's disease (HD). It was established that mice transgenic for exon 1 of the human HD gene carrying (CAG)115 to (CAG)156 repeat expansions show clear neuronal intranuclear inclusions (NIIs) before developing a neurological phenotype. Neurons within the striatum of mice transgenic for the HD mutation contain an NII that contains the proteins htt and ubiquitin.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Exon 1 of the HD gene with an expanded CAG repeat is sufficient to cause a progressive neurological phenotype in transgenic mice
Article Abstract:
Studies using mice transgenic for the 5' end of the human Huntington's disease (HD) gene carrying expanded CAG repeats have shown that the expanded CAG repeats are within exon 1 of the genomic fragment. The mice exhibited neurological disorders that are characteristic to HD such as epileptic seizures, tremor, motor abnormalities, cerebellar dysfunctions, voluntary movement disorders, as well as emotional disorders.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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- Abstracts: Distinct parasegmental and imaginal enhancers and the establishment of the expression pattern of the Ubx gene
- Abstracts: Physical and genetic map of the genome of Campylobacter upsaliensis. Campylobacter upsaliensis exerts a cytolethal distending toxin effect on HeLa cells and T lymphocytes
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