Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast
Article Abstract:
A study of the molecular features of the silencing complex in Saccharomyces cerevisiae through an analysis of the interactions between H3 and H4 histones and other silencing proteins (SIRs) reveals that the interaction between SIR3 and SIR4, and H3 and H4 occurs at the silencing domains of the histone N-termini. The in vitro interactions are affected by mutations in SIR-3 and -4. Immunofluorescence studies with antibodies against SIR3 and RAP1 indicate that the perinuclear positioning of yeast telomeres and the binding of SIR3 with telomeric chromatin need the N-termini of H3 and H4.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast
Article Abstract:
A study was conducted to determine the effects of the release of telomeric Ku (yKu) and nucleosome-binding silent information regulator (SIR) proteins under conditions of induced DNA damage. Results suggest that the relocalization of yKu and the SIR proteins from telomeres' subtelomeric domain of heterochromatin are mediated by RAD9-dependent response to DNA damage. It was also observed that yKu was immediately recruited to sites of double-strand double-strand cleavage while SIR proteins are translocated to the near cleavage sites.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Yeast heterochromatin: regulation of its assembly and inheritance by histones
Article Abstract:
Scientific papers focusing on the role of histones and histone acetylation in the assembly and inheritance of yeast heterochromatin are evaluated. These studies show that acetylation may allow the initiation and spread of heterochromatin via the regulation of protein-protein interaction. The involvement of heterochromatin in chromosomal segregation, genetic repression and cellular senescence are also proposed.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Left and right ventricular contributions to the formation of the interventricular septum in the mouse heart
- Abstracts: Isolation and characterization of Vibrio tubiashii outer membrane proteins and determination of a toxR homolog
- Abstracts: A deubiquitinating enzyme interacts with SIR4 and regulates silencing in S. cerevisiae. Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae
- Abstracts: Hereditary multiple malformation in Japanese quail: a possible powerful animal model for morphogenetic studies