Isolation and characterization of Vibrio tubiashii outer membrane proteins and determination of a toxR homolog

Article Abstract:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, N-terminal amino acid sequencing and PCR analyses are used for characterizing the outer membrane proteins (OMPs) expressed by Virbio tubiashii under different environmental growth conditions. The results have disclosed the presence of a 38- to 40-kDa OmpU-like protein and ompU gene, a maltoporin-like protein, several novel OMPs and a regulatory toxR homolog.

Science & research, Usage, Technology application, Polymerase chain reaction, Gel electrophoresis, Gene expression, Amino acid sequence, Amino acid sequencing

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Perkinsus marinus extracellular protease modulates survival of Vibrio vulnificus in Eastern oyster (Crassostrea virginica) hemocytes

Article Abstract:

Research was conducted to examine the in vitro effects of Perkinsus marinus serine protease treatment on the uptake and intracellular survival of Vibrio vulnificus within oyster hemocytes through the use of a gentamicin internalization assay. Results indicate that the serine protease yielded by P marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V vulnificus.

Proteases, Vibrio, Oysters, Blood cells

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Rugosity in Grimontia hollisae

Article Abstract:

The rugose colony phenotype in Grimontia hollisae was characterized by wrinkled colonies producing copious amounts of exopolysaccharide. The results indicate that the rugose exopolysaccharide consists partially of N-acetylglucosamine and mannose, produced significantly more biofilm material than smooth colonies and also survived more than smooth colonial variants.

Analysis, Physiological aspects, Genetic aspects, Flow cytometry, Bacteria, Pathogenic, Pathogenic bacteria, Nucleotide sequencing

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