Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
Article Abstract:
Researchers report the discovery of benzylsuccinate synthase activity in a methanogenic culture. Until this report, this newly discovered enzyme had only been described in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria. The enzyme attaches the methyl carbon of toluene to the double bond of fumarate to form benzylsuccinate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Identification and expression of benzylsuccinate synthase genes in a toluene-degrading methanogenic consortium
Article Abstract:
A study was conducted to identify benzylsuccinate synthase (BSS) gene sequences in a highly enriched toluene degrading methanogenic consortium. The differential transcription of bssA-2, combined with the bioinformatic analysis of the sequence provides strong evidence that bssA-2 encodes an enzyme that shares the same catalytic function as those encoded by BssA sequences from the nitrate- and iron-reducing microorganisms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Benzylsuccinate formation as a means of anaerobic toluene activation by sulfate-reducing strain PRTOL1
Article Abstract:
Microbiological research using permeabilized, toluene-grown microbial cells of sulfate-reducing strain PRT0L1 suggests that benzylsuccinate formation from toluene-fumarate catalysis occurs in microorganisms other than Proteobacteria denitrifiers. Anaerobic toluene activation by fumarate addition occurs in widely differing bacterial groups.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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