Identification of the inducing agent of the 2,4-dichlorophenoxyacetic acid pathway encoded by plasmid pJP4
Article Abstract:
Dichloromuconate is the inducing agent of the 2,4-dichlorophenoxyacetic acid (2,4-D) pathway of Alcaligenes eutrophus JMP134 (pJP4) plasmid. The identification is based on the analysis of beta-galactosidase gene lacZ transcriptional fusions with tfd structural genes in the presence and absence of 2,4-D. Fusions of individual genes help in reporter and disrupter activities of gene expression. Increased reporter activities are evident in fusions occurring in genes that encode enzymes, which function after the production of the inducing intermediate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Rapid evolution of novel traits in microorganisms
Article Abstract:
Researchers designed a plasmid that can increase the mutation frequency of a bacterium by 20- to 4,000-fold. Once the desired trait is achieved, the plasmid can be removed, returning the bacterium to its normal mutation rate. This technique could be used to develop bacteria that can biodegrade high concentrations of metabolites and solvents.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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