Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50
Article Abstract:
The generation of specific polyclonal antibodies against chemically synthesized enterocin Q (EntQ) and the C- and N-terminal fragments of enterocin L50A (EntL50A) and enterocin L50B (EntL50B) as well as EntQ, respectively, are described. The analysis has demonstrated the suitability of an immunochemical strategy based on the use of bacteriocin-specific polyclonal antibodies and the results have shown a strong influence of temperature on EntL50, EntP and EntQ production by Enterococcus faecium L50.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from enterococcus faecium P13 with broad antimicrobial spectrum
Article Abstract:
A novel pediocin-like bacteriocin is isolated, purified and analyzed. The new bacteriocin is called enterocin P and is isolated from a dry fermented Spanish sausage. It is produced by Enterococcus faecium and exhibits a broad antimicrobial spectrum including microorganisms that cause food spoilage. Enterocin P is sec-dependent. The biochemical, molecular and functional properties of this bacteriocin are presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity
Article Abstract:
The locations of the genetic determinants for enterocin L50 (EntL50A and EntL50B), enterocin Q (EntQ) and enterocin P (EntP) were determined in the multiple bacteriocin producer Enterococcus faecium strain L50. It was demonstrated that 2,371-bp fragment from pCIZ2 was sufficient for EntQ production, EntqB was involved in ABC transporter-mediated EntQ secretion while EntqC confered immunity to bacteriocin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Isolation and characterization of temperate bacteriophages of Clostridium difficile
- Abstracts: Intestinal bacterial communities that produce active estrogen-like compounds enterodiol and enterolactone in humans
- Abstracts: Genotypic and physiological characterization of Saccharomyces boulardii, the probiotic strain of Saccharomyces cerevisiae
- Abstracts: Regulation of the Xenopus labial homeodomain genes, HoxA1 and HoxD1: activation by retinoids and peptide growth factors
- Abstracts: Male facial appearance signals physical strength to women. Endurance running and digit ratio (2D:4D): Implications for testosterone effects on running speed and vascular health