Importance of tetrahydofolate and ATP in the anaerobic O-demethylation reaction for phenylmethylethers
Article Abstract:
The requirements and location ofthe anaerobic phenylmethylether O-demethylating activity in Acetobacterium woodii were determined. The results showed that the anaerobic O-demethylating activity required tetrahydrofolate and adenosine triphosphate. The enzyme activity was not membrane associated, and subcellular fraction assays showed that the cytoplasmic fraction was 34% as active as the intact cells. No requirements for pyridine nucleotides were detected, suggesting that the anaerobic O-demethylating activity is mechanistically distinct from the action of aerobic hydoxylating enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Reciprocal isomerization of butyrate and isobutyrate by the strictly anaerobic bacterium strain WoG13 and methanogenic isobutyrate degradation by a defined triculture
Article Abstract:
Isomerization of butyrate and isobutyrate was reported for the first time in a strictly anaerobic bacterium, WoG13. Experiments on cell extracts and nuclear magnetic resonance studies proved that the isomerization reaction involved the action of coenzyme A transferases to activate the substrate, and mutases to rearrange the organic moeity. Coenzyme B(sub 12) was required in the reaction. A triculture composed of WoG13, Syntrophomonas wolfei and Methanospirillum hungatei completely degraded isobutyrate via butyrate to acetate and methane.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Enumeration of acetogens by a colorimetric most-probable-number assay
Article Abstract:
The titanium(III)-based colorimetric most-probable-number assay is an effective, convenient and consistent method for enumeration of anaerobic O demethylating acetogens in environmental samples. Interaction of the hydroxyaromatic products of anaerobic O demethylation with titanium(III) leads to the formation of yellow color in the medium. Anaerobic O demethylation of vanillate, a methoxylated aromatic substrate, to protocatechuate is also evident. The environmental samples differ in the population titers of O demethylating bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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