Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag
Article Abstract:
A tag consisting of three lysines alternating with three glycines is added to the C-terminal end of the beta chain of penicillin G acylase (PGA) in order to facilitate access of the substrate to the active site of PGA. The modification has improved the immobilization efficiency of PGA on glyoxyl agarose and the catalytic properties of the PGA derivative, even though it impaired the posttranslational steps of overexpressed protein maturation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Genetic transformation in freshwater: Escherichia coli is able to develop natural competence
Article Abstract:
The ability of Escherichia coli to develop natural competence in freshwater was investigated by examining its transformation in associated environments. Transformants were detected in waters containing calcium ions at concentrations of as low as 1 to 2 mM. Analysis of the transformation frequency of samples revealed a significant homology with those for other gram-negative bacteria that exhibit natural competence.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Pathotyping Escherichia coli by using miniaturized DNA microarrays
Article Abstract:
The sensitive and specially miniaturized DNA microarrays having oligonucleotide probes are developed for the detection of determinants in pathotyping Escherichia coli. Various pathotypes responsible for infection are found.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Degradation of carboxy-terminal-tagged cytoplasmic proteins by the Escherichia coli protease HflB (FtsH). Epidermal detachment, desmosomal dissociation, and destabilization of corneodesmosin in Spink5(super -/-) mice
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