In situ gene expression in mixed-culture biofilms: evidence of metabolic interactions between community members

Article Abstract:

The compartmentalization of specific gene expression was examined by studying microbial communities growing in laboratory-based flow chambers. The study focused on Pseudomonas putida and Acinetobacter sp. strain C6, which have been found to be able to degrade benzyl alcohol and to have degradative pathways similar to that found on the TOL plasmid. Specific gene expression in the toulene-degrading mixed community biofilm was investigated by forming genetic fusions between the relevant promoters and the gfp gene, and then introducing the engineered strains into the microbial community. The results are discussed.

Genetic aspects, Microbial metabolism, Gene expression, Promoters (Genetics), Pseudomonas putida

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Establishment of new genetic traits in a microbial biofilm community

Article Abstract:

A flow chamber biofilm community consisting of Pseudomonas putida, Acinetobacter sp. and a D8 isolate was used in the investigation of the horizontal and vertical TOL plasmid transfer. P. putida is the only species capable of acting as a recipient of the plasmid. The horizontal plasmid transfer frequency is low and the build-up of the number of organisms containing the plasmid can be accounted for by vertical plasmid transfer.

Plasmids, Bacterial transformation, Microbial genetics

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Impact of nutrient composition on a degradative biofilm community

Article Abstract:

A microbial population was cultivated in flow cells with 2,4,6-trichlorobenzoic acid (2,4,6-TCB) as the only carbon and energy source and was studied with scanning confocal laser microscopy and fluorescent molecular probes. This bacterial community showed a characteristic architecture, including a basal cell layer and conspicuous mounds of bacterial cells and polymer occurring at 20- to 200-micron intervals.

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