Inhibition of precardiac mesoderm cell proliferation by antisense oligodeoxynucleotide complementary to fibroblast growth factor-2 (FGF-2)
Article Abstract:
The effects of antisense oligodeoxynucleotide (ODN) complementary to fibroblast growth factor-2 (FGF2) on embryo precardiac anterior lateral plate mesoderm cells were examined in a study aimed to determine FGF-2's role during cardiogenesis. Layers of proliferative cells expressing sarcomeric alpha-actin and displaying contractility were formed from the monolayer after two days of culture. Proliferative ability and contractility were inhibited upon addition of ODN which was complementary to FGF-2 mRNA's second exon. Adding recombinant human FGF-2 protein eliminated this inhibitory effect. These suggest that FGF-2 plays an important role in the autocrine regulation of cardiac myocyte proliferation and function in embryos.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1993
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Both basic fibroblast growth factor and ciliary neurotrophic factor promote the retention of polyneuronal innervation of developing skeletal muscle fibers
Article Abstract:
A study was conducted to determine the effects of basic fibroblast growth (bFGF) factor and ciliary neurotrophic factor (CNTF) on loss of polyneuronal innervation in rat muscles. Intracellular recording analysis of the muscle fibers revealed an increase in percentage of polyneurally innervated fibers in muscles injected with bFGF and CNTF, compared to saline-injected muscles. bFGF-injected muscles exhibited 60% polyneuronal innervation, while CNTF-injected muscles exhibited 40% innervation. These results suggest that both bFGF and CNTF exert long-lasting effects on neuromuscular transmission.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
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Leukemia inhibitory factor and ciliary neurotrophic factor regulate expression of muscarinic receptors in cultured sympathetic neurons
Article Abstract:
Cultured sympathetic neurons of the neonatal rat superior cervical ganglion were studied with respect to regulation of muscarinic receptor expression. The effects of the leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) on receptor expression were also examined. The results demonstrate that LIF and CNTF regulate receptor expression and neurotransmitter development. Furthermore, its potential role in synaptogenesis in the developing nervous system was discussed.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1993
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