Insertional mutagenesis of the maize P gene by intragenic transposition of Ac

Article Abstract:

The maize P gene regulates the biosynthetic pathway leading to the formation of flavonoid-derived red pigments in floral organs, while it P-rr allele specifies red color. The P gene was sequenced and characterized using Ac insertion mutagenesis. Results showed that Ac inserts nonrandomly, and is distributed in both 5' and 3' ends of P. All of the Ac insertions affected cob glume and pericarp pigmentation, indicating that P-rr contains a single gene for its phenotypic effect. Characterization of P by insertion mutagenesis showed similar results to those obtained by molecular techniques.

Usage, Transposons, Mutagenesis, Plant pigments

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A new property of the maize B chromosome

Article Abstract:

The B chromosome is a nonessential extrachromome, which in maize is maintained by accumulation mechanisms involving mitotic nondisjunction. By studying effects of B deletionsduring TB-9Sb translocations, the maize B chromosome was further characterized.The results showed that maize chromosome B suppresses meiotic loss through one proximal and one distal region on the B chromosome. Meiotic loss is controlled by affecting chromosome movement in anaphase I. It is proposed that the B chromosomes of many species display similar properties.

Meiosis, Extrachromosomal DNA

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The heterochronic Teopod1 and Teopod2 mutations of maize are expressed non-cell-autonomously

Article Abstract:

Teopod1 and Teopod2 are mutations in corn which promote the expression of traits observed in the a juvenile phase at the adult stage. The cell autonomy of the two dominant mutations was analyzed to study the basis of the phenotypes resulting from the mutations. One standard and two nonstandard procedures were employed in the analysis. Results indicate that both mutations are non-cell-autonomous.

Plant mutation

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