Integrated mapping and sequencing of a 115 kb DNA fragment from Bacillus subtilis: sequence analysis of a 21 kb segment containing the sigL locus
Article Abstract:
A novel chromosome mapping technique was developed based on a low coverage shotgun approach and directed sequencing steps. The method was utilized for nucleotide sequencing of the 115 kb chromosomal fragment of the Bacillus subtilis genome. Low coverage shotgun approach for the mapping of the 115 kb chromosomal fragment indicated the presence of 21 open reading frames that belong to seven transcription units. The G+C content of the segment is greater than 47% and contains genes that regulate the synthesis of exopolysaccharide.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene
Article Abstract:
The region from the prkA to the addAB located at the 83 degree and 97 degree positions respectively contains 171,812 base pairs and accounts for 4.1% of the entire genome of Bacillus subtilis. This region was cloned, sequenced and analyzed as a part of the genome sequencing project for B. subtilis. Genetic analyses revealed that this chromosome region contains a number of dysfunctional genes. There are also sequences coding for a number of transporter proteins and the glyB marker.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area
Article Abstract:
The 27 kp deoxyribonucleic acid (DNA) fragment that is located near the nprE gene of Bacillus subtilis was cloned and systematically sequenced. Sequencing of the DNA clones representing the ampS-nprE region of Bacillus subtilis genome indicated the presence of 23 open reading frames (ORFs). Furthermore, 17 of the ORFs utilize the ATG start codons while three ORFs have GTG start codons. The sequenced region near the nprE gene and the pyr operon was assigned as adeC and strB genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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