In vitro (super)23 Na nuclear magnetic resonance study of maintanance of a sodium gradient in the ruminal bacterium Fibrobacter succinogenes S85
Article Abstract:
Research has been conducted on the sodium gradients in the Fibrobacter succinogenes resting cells. The use of the (super)23 Na nuclear magnetic resonance in measuring these gradients is described, and the results indicate the modification of glucose metabolism and inability to maintain the sodium gradient due to Na (super)+ ions depletion.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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13C and 1H nuclear magnetic resonance study of glycogen futile cycling in strains of the genus Fibrobacter
Article Abstract:
Three strains of Fibrobacter succinogenes and one strain of Fibrobacter intestinalis were analyzed using C and H nuclear magnetic resonance analysis. The four strains revealed a reversal of glycolysis at the triose phosphate level and similar metabolic pathways. Results also revealed that 12% to 16% of the glucose entering the metabolic pathway originated from prestored glycogen. Overall, the four strains had the same carbon metabolism characteristics despite genetic differences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Interactions between carbon and nitrogen metabolism in Fibrobacter succinogenes S85: a 1H and 13C nuclear magnetic resonance and enzymatic study
Article Abstract:
13C and 1H nuclear magnetic resonance (NMR) were employed to examine the effect of the presence of ammonia on (1-(super 13)C)glucose metabolism in the rumen fibrolytic bacterium Fibrobacter succinogenes S85. Results demonstrate the activity of a reversible glutamate dehydrogenase in F succinogenes S85. GLDH and alanine dehydrogenase were the main pathways of ammonia assimilation. Results obtained by 13C-NMR and 13C-filtered spin-echo difference 1H-NMR spectroscopy indicate a modulation of carbon metabolism by ammonia in F succinogenes S85.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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