Intergeneric transfer of conjugative and mobilizable plasmids harbored by Escherichia coli in the gut of the soil microarthropod Folsomia candida (collembola)
Article Abstract:
An examination of the soil microarthropod Folsomia candida revealed a high density of bacterial cells in its gut. In an effort to determine whether such cells promote gene transfer in microbial communities, experiments were conducted to determine if they act as plasmid recipients. To do this, F. candida in microcosms were fed Escherichia coli strains bearing self-transferable, mobilizable and nonmobilizable plasmids. The ability of the bacterial cells to intergenetically transfer the plasmids suggests that the F. candida gut can be used as hot spot for soil genetic transfers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Direct detection of recombination gene expression by two genetically engineered yeasts in soil on the transcriptional and translational levels
Article Abstract:
The expression of recombinant genes by genetically engineered yeasts in soil can be determined by studying the gene transcripts and the encoded proteins. The mRNA, produced by the aprotinin encoding gene in the Saccharomyces cerevisiae WHL292 and Hansenula polymorpha LR9-Apr4 strains, is identified by lysing bacterial cells in the soil followed by reverse transcriptase PCR. The aprotinin is identified by enzyme-linked immunosorbent assay. Studies show that the recombinant gene is expressed during the first four days of innoculation in sterile and nonsterile soil.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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