Intra-specific diversity within Pasteurella trehalosi based on variation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins

Article Abstract:

Variations of capsular polysaccharide and lipopolysaccharide (LPS) and outer-membrane protein (OMP) profiles in sixty isolates of Pasteurella trehalosi are analyzed to determine intra-specific diversity in the bacterial pathogen, which is responsible for serious systemic infection and pneumonia in sheep. Indirect haemagglutination assay is used to determine capsular polysaccharide serotypes while SDS-PAGE analysis is used to compare LPS and OMP profiles. Results indicate that a combination of capsular serotyping and LPS and OMP typing is useful for determining diversity in P. trehalosi.

Biodiversity, Endotoxins, Membrane proteins, Bacteria, Pathogenic, Pathogenic bacteria, Species diversity

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Mutants of Rhizobium tropici strain CIAT899 that do not induce chlorosis in plants

Article Abstract:

Rhizobium tropici type B strains induce foliar chlorosis if applied to seeds of symbiotic host and non-host dicotyledonous plants. CT4812, a Tn5-induced mutant of R. tropici strain CIAT899, unable to induce chlorosis, has been isolated. The Tn5 insertion was found to be located in a similar gene to glnD, encoding uridylyltransferase/uridylyl-removing enzyme in enteric bacteria. A mutation in mviN, the Salmonella typhimurium virulence gene, had no effect on CIAT899's ability to induce chlorosis in bean plants.

Chlorosis (Plants)

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Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain GRH2: major requirement of lipopolysaccharide for successful invasion of Acacia nodules and host range determination

Article Abstract:

A study of two transposon Tn5-activated mutants of the broad-host-range Rhizobium sp. GRH2, GRH2-14 and GRH2-50, reveals a deficiency in the synthesis of smooth lipopolysaccharides (LPS) by these enzymes. UDPglucose 4-epimerase activity, which is essential for the synthesis of LPS, is lacking in the exoB mutant GRH2-50, leading to LPS synthesis deficiency. There is no homology between any rhizobial strain DNA and GRH2-14 mutant DNA, indicating that GRH2-14 mutant locus denotes a new Acacia rhizobia lps gene.

Analysis, Microbial mutation

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