Invasion of the nucleotide snatchers: Structural insights into the mechanism of G protein GEFs
Article Abstract:
Replacement of GDP by GTP in the active G protein site requires the help of a guanine nucleotide exchange factor (GEF). GEFs are structurally diverse, and the structures of two new complexes have recently been elucidated, Ras with the CDC25 domain of Sos1, and ARF1 with the Sec7 domain of the S. cerevisiae protein Gea2. The new structures, together with previously determined GDP and GTP bound complexes of G proteins, offer an insight into the mechanism of activation by nucleotide exchange factors.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
The structure of the G protein Heterotrimer G(sub i-alpha)-beta(sub 1)-gamma (sub 2)
Article Abstract:
The G protein heterotrimer formed by the alpha(sub i1), beta(sub 1) and gamma(sub 2) subunits is an asymmetrical molecule which is stabilized by interactions between the alpha and beta subunits. These interactions are necessary for the formation of the heterotrimer. The beta subunit forms a propeller domain containing seven motifs. There are two nonoverlapping contact sites between the alpha and beta subunits and an extended interface between beta and gamma.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Structure of RGS4 bound to AIF4-activated Gialpha1: stabilization of the transition state for GTP hydrolysis
Article Abstract:
A study of the crystal structure of the RGS protein RGS4 complexed with Gialpha1 was conducted. The core domain binds to the three switch regions of Gialpha1 but does not contribute catalytic residues that interacts directly with either GDP or AIF4-. The findings indicate that RGS4 seems to catalyze rapid hydrolysis of GTP primarily through the stabilization of the switch regions of Gialpha1.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Environmental transmission of a sulfur-oxidizing bacterial gill endosymbiont in the tropical lucinid bivalve Codakia orbicularis
- Abstracts: DAX1 mutations map to putative structural domains in a deduced three-dimensional model. Gene tests: integrating genetic services into patient care
- Abstracts: mtDNA history of the Cayapa Amerinds of Ecuador: detection of additional founding lineages for the Native American populations
- Abstracts: Systematic sequencing of the Bacillus subtilis genome: progress report of the Japanese group. Determination of a 17484 bp nucleotide sequence around the 39 degree region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs
- Abstracts: Aspects of the life history and foraging ecology of the endangered Akiapolaau. Demography and movements of Apapane and Iiwi in Hawaii