Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep
Article Abstract:
A T4-like bacteriophage CEV1 is isolated from sheep that are resistant to colonization by Escherichia coli O157:H7 and is characterized in order to determine its potential to control the levels of the pathogen in sheep. It is observed that CEV1 efficiently infects E.coli O157:H7 grown both aerobically and anaerobically in vitro, and reduces sheep intestinal E.coli levels within 2 days of administration of an oral dose in vivo.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Development and validation of a real-time PCR method to quantify Rumen protozoa and examination of variability between Entodinium populations in sheep offered a hay-based diet
Article Abstract:
A study was conducted on the design of PCR and real-time PCR primers for the 18S rRNA gene of rumen protozoa, and their specificities were also tested against a range of rumen microbes and protozoal groups. It was observed that the numbers of Entodinium cells calculated using real-time PCR were higher than microscopic estimations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Molecular diversity of rumen methanogens from sheep in Western Australia
Article Abstract:
The determination of the molecular diversity of methanogens in the ovine rumen in Western Australia is examined. The compositions of the methanogen populations in pasture-grazed sheep are compared with the compositions of the methanogen populations in sheep fed forage-based diets.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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