Serotyping, stx(sub 2) subtyping, and characterization of the locus of enterocyte effacement island of Shiga toxin-producing Escherichia coli and E. coli O157:H7 strains isolated from the environment in France

Article Abstract:

Shiga toxin-producing Escherichia coli (STEC) and E. coli O157:H7 strains that were isolated from the environment in France were subjected to serotyping, stx(sub 2) subtyping and the characterization of the locus of enterocyte effacement island was conducted. The results indicate that the environment is a reservoir for STEC pathogens and emphasizes on the need for appropriate handling and use of manure, slurry or sewage sludge for preventing the contamination of the environment and food by (STEC).

Science & research, Causes of, Contamination, Ecology, Pathogenic microorganisms, Sewage sludge

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Heterogeneity of Shiga toxin-producing Escherichia coli strains isolated from hemolytic-uremic syndrome patients, cattle, and food samples in central France

Article Abstract:

Stx(sub 2)-restriction fragment length polymorphism, stx(sub 2) variant, and plasmid profile analysis is as accurate as pulsed-field gel electrophoresis for differentiating Shiga-toxin-producing strains of E. coli. In central France, many of the strains isolated from food, animals, and humans with hemolytic-uremic syndrome belonged to the O91 and OX3 serogroups.

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Localization of the insertion site and pathotype determination of the locus of enterocyte effacement of Shiga toxin-producing Escherichia coli strains

Article Abstract:

Research shows that the locus of enterocyte effacement (LEE) pathogenicity island is inserted in the vicinity of selC, pheU, or pheV tRNA gene in Escherichaia coli strains, suggesting the genetic diversity of LEE phathotypes and LEE insertion sites.

Genetic aspects, Biological diversity, Biodiversity, Bacteria, Pathogenic, Pathogenic bacteria, Microbial toxins

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