Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production
Article Abstract:
The isolation and characterization of 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas sp. strain F113 was described. An assay based on high-pressure liquid chromatography was developed for quantitative analysis of the DAPG in both culture media and soil. Under the assay conditions, it was shown that DAPG production was optimum at 12 degrees centigrade. The types of carbon sources also affected DAPG production with sucrose, mannitol and fructose providing the highest yields. In addition, a low ratio of culture volume to surface area in a growth container was critical for maximum growth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Characterization of fluorescent siderophore-mediated iron uptake in Pseudomonas sp. strain M114: evidence for the existence of an additional ferricsiderophore receptor
Article Abstract:
A mutant of Pseudomonas specie strain M114 lacking the outer membrane receptor for ferric pseudobactin M114 was constructed and characterized. This mutant showed lack of an 89- kilodalton protein in the outer membrane and incapability in accumulating ferric pseudobactin M114. Cross-feeding assays showed the presence of an additional ferric pseudobactin receptor for the uptake of iron complexed to pseudobactin MT3A that strain M114 does not produce.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens
Article Abstract:
Certain species of the fluorescent Pseudomonas have been found to have biological control of plant root pathogens. The production of siderophores or iron-binding compounds and antibiotic compounds are inhibitory to the plant root pathogens. However, it has been found out that effective biocontrol is dependent on root colonization. Further studies are needed to fully realize the commercial viability of the process.
Publication Name: Microbiological Reviews
Subject: Biological sciences
ISSN: 0146-0749
Year: 1992
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- Abstracts: Impact of 2,4-diacetylphloroglucinol-producing biocontrol strain Pseudomonas fluorescens F113 on intraspecific diversity of resident culturable fluorescent pseudomonads associated with the roots of field-grown sugar beet seedlings
- Abstracts: Transformation of 2,4,6-trinitrotoluene by Pseudomonas pseudoalcaligenes JS52. Oxidative transformation of aminodinitrotoluene isomers by multicomponent dioxygenases
- Abstracts: Degradation of trichloroethylene by Pseudomonas cepacia G4 and the constitutive mutant strain G4 5223 PR1 in aquifer microcosms
- Abstracts: Isolation of a Pseudomonas solanacearum-specific DNA probe by substraction hybridization and construction of species-specific oligonucleotide primers for sensitive detection by the polymerase chain reaction
- Abstracts: Intestinal colonization potential of turbot (Scophthalmus maximus)- and dab (Limanda limanda)-associated bacteria with inhibitory effects against Vibrio anguillarum