Isolation of a Pseudomonas solanacearum-specific DNA probe by substraction hybridization and construction of species-specific oligonucleotide primers for sensitive detection by the polymerase chain reaction
Article Abstract:
A Pseudomonas solanacearum-specific DNA probe was isolated by subtractive hybridization. The probe, designated PS2096, is highly specific to P. solanacearum, although it did not hybridize to three strains, namely, GMI1336, UW119 and UW373. However, these strains do not possess the genes for pathogenicity and hypersensitive response. The probe was sequenced, and based on the results, oligonucleotide primers were developed for use in polymerase chain reaction assays. This would constitute a highly sensitive and rapid method for the detection of P. solanacearum.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods
Article Abstract:
A polymerase chain reaction-based method for the rapid detection of Listeria monocytogenes was developed. The procedure involved the use of primers based on a unique region in the 16S rRNA sequence of L. monocytogenes. The procedure resulted in shorter denaturing and annealing times, as well as more rapid transitions, to detect as few as 2 to 20 colony forming units of L. monocytogenes in inoculated food samples. Further analysis showed that the procedure is highly specific for L. monocytogenes, although soft cheese produced interference.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Identification of Brucella spp. by using the polymerase chain reaction
Article Abstract:
Two oligonucleotide probes for the identification of Brucella spp. were developed. The probes were derived from primer sequences of the 16S rRNA of B. abortus. Using the probes as primers for polymerase chain reaction resulted in the amplification of specific Brucella DNA fragments. Tests on other species, including genetically related species, were negative for amplification. The results show that the two probes may be used for the quick and reliable detection of Brucella spp.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar enteritidis
- Abstracts: Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR
- Abstracts: Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone. The mechanism of Hsp90 regulation by the protein kinase-specific cochaperone p50
- Abstracts: Purification and characterization of an endoglucanase from Streptomyces lividans 66 and DNA sequence of the gene
- Abstracts: Unstable expression and thermal instability of a species-specific cell surface epitope associated with a 66-kilodalton antigen recognized by monoclonal antibody EM-7G1 within serotypes of Listeria monocytogenes grown in nonselective and selective broths