Isolation of an IHF-deficient mutant of a Pseudomonas aeruginosa mucoid isolate and evaluation of the role of IHF in algD gene expression
Article Abstract:
The integration host factor (IHF) has been shown to bind the Pseudomonas aeruginosa algD promoter, which is one of the gene clusters involved in alginate biosynthesis. To determine its role in algD gene expression, IHF-deficient Escherichia coli strain BL21(DE3) was used as a host strain to overproduce P. aeruginosa IHF, and binding of P. aeruginosa IHF to algD promoter was investigated. Insertional inactivation of an himA, an IHF gene, resulted in a three- to fourfold decrease in algD expression under conditions of high salinity and nitrogen limitation. These results indicate that himA activation is essential for algD expression.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Effects of growth-inhibitory concentrations of copper on alginate biosynthesis in highly mucoid Pseudomonas aeruginosa
Article Abstract:
The effects of growth-inhibitory concentrations of copper on alginate production in Pseudomonas aeruginosa at the transcriptional level and the degree of polymerization were investigated. The decreased activity of all the four enzymes essential for GDP-mannuronic acid synthesis in cells grown in CuCl2-supplemented medium corresponds with the decrease of alginate production by copper-stressed cells. Findings are consistent with the proposed linkage between the control of alginate production and the global regulation involved in oxidative stress responses.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung
Article Abstract:
A study was conducted to analyze the influence of activated polymorphonuclear leukocytes and their release of toxic oxygen by-products in the cystic fibrosis lung environment in the generation and/or selection of mucoid variants during inflammatory responses to Pseudomonas aeruginosa. Results indicated that polymorphonuclear leukocytes and their oxygen radicals can promote phenotypic and genotypic changes that are typical of the intractable form of P.aeruginosa in the cystic fibrosis lung.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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