Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity
Article Abstract:
Length heterogeneity analysis by polymerase chain reaction (LH-PCR) was employed to determine the composition of bacterioplankton populations from a water sample from off the Oregon coast. Length variability in small-subunit rDNAs of bacterial strains were examined to trace the phylogenetic origins of the domains amplified by LH-PCR. Findings showed that the relative gene frequencies are highly reproducible when PCR production concentrations are restricted to relatively low values. However, at high concentrations, the kinetic bias causes large distortions in gene frequencies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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A novel delta-subdivision proteobacterial lineage from the lower ocean surface layer
Article Abstract:
A small-subunit ribosomal RNA (16S rRNA) gene lineage (SAR324) related to the delta subdivision of the class Proteobacteria (DP) was found in a 16S rRNA gene clone library prepared from a water sample obtained from 250 m in the western Sargasso Sea. Phylogenetic analysis revealed that SAR324 was most closely related to the DP, although it had no specific affinity to any DP 16S rRNA genes in databases.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Representative freshwater bacterioplankton isolated from Crater Lake, Oregon
Article Abstract:
Isolation and characterization of bacterioplankton representatives of typical, abundant phylotypes in Crater Lake is described. The ultraoligotrophic status of Crater Lake is likely to favor the survival of organisms able to switch between heterotrophy and phototrophy as light availability and organic carbon levels fluctuate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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