Lack of correlation between trehalose accumulation, cell viability and intracellular acidification as induced by various stresses in Saccharomyces cerevisiae
Article Abstract:
Trehalose accumulation after stress is investigated in a wild type of the yeast Saccharomyces cerevisiae and in a pma1-1 mutant under conditions of elevated temperature, addition of ethanol, acidification of the medium and addition of decanoic acid. Stress induced accumulation of trehalose but a careful investigation of the metabolic response under stress was unable to elucidate a common mechanism of response generation. It is postulated that changes in the plasma membrane brought about by the stressful regime is responsible for the observed trehalose accumulation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Effects of various types of stress on the metabolism of reserve carbohydrates in Saccharomyces cerevisiae: genetic evidence for a stress-induced recycling of glycogen and trehalose
Article Abstract:
An analysis of the transcriptional induction and metabolic activities of Saccharomyces cerevisiae genes involved in glycogen and trehalose accumulation in response to various types of stress was conducted to determine the physiological role of stress-responsive elements present in the promoter of genes. Results show that one of the major effects of stress in yeasts is to induce a wastefulof energy by increasing the recycling of these molecules. It was also found that transcriptional induction of STE-controlled genes was abolished at 40 degrees Celsius.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, is involved in the transcriptional control of chitin synthase genes
Article Abstract:
A study was conducted to show that KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, influences the transcriptional control of chitin synthase genes. The LiAc technique was utilized to carry out the transformation of calcofluor-white hypersensitive mutants. Experimental results indicated that the suppressor supports a regulatory function in chitin deposition and in cell wall assemblies.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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