Linking toluene degradation with specific microbial populations in soil
Article Abstract:
Toluene degradation has been linked with specific microbial populations in soil. Traditional culture-based approaches and phospholipid fatty acid (PLFA) and C-PLFA methods not affected by biases associated with laboratory cultivation were used to identify the indigenous populations responsible for disappearance of toluene in Yolo silt loam, a surface soil. PLFA analysis of a soil microbial community was coupled with (super.13)C isotope tracer analyses to measure the community's response to addition of 35 micrograms of (super.13)toluene/ml of soil solution. Results showed coupling the methods is effective for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Isolation of saprophytic basidiomycetes from soil
Article Abstract:
The isolation of saprophytic basidiomycetes from soil involves selective inhibitors, an indicator substrate and soil particle washing. The washed organic particles are plated on a medium containing lignin, guaiacol and benomyl to reduce mold growth and allow detection of basidiomycetes producing laccase. The method is useful for the investigation of biodiversity of soil basidiomycetes and gives organisms for bioremediation of soils contaminated with pesticides.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Diversity of basidiomycetes in Michigan agricultural soils
Article Abstract:
The communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellog Biological Station Long-Term Ecological Research site near Battle Creek, Michigan are analyzed. Results reveal that only 24 of 241 species had 99% or greater sequence similarity to named reference sequences in GenBank, and several clades with multiple 'species' could not be identified at the genus level by phylogenetic comparisons with named sequences.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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- Abstracts: Genetic variation within lotic population of Janthinobacterium lividum. Genetic structure of a lotic population of Burkholderia (Pseudomonas) cepacia
- Abstracts: Fungal degradation of lipophilic extractives in Eucalyptus globulus wood. Lignin-derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes
- Abstracts: Photosynthate partitioning and fermentation in hot spring microbial mat communities. Cultivation of aerobic chemoorganotrophic proteobacteria and gram-positive bacteria from a hot spring microbial mat
- Abstracts: Influence of incubation temperature on the microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in two freshwater sediments