Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura
Article Abstract:
The intraembryonic precursor source in the paraaortic splanchnopleura (P-Sp) and aorta-gonad-mesonephros is investigated using a culture system of isolated mouse Sp. Dissection of Sp is made prior to circulation. Hemopoietic intraembryonic precursors are observed even at the presomite stages or at 7.5-8 days postcoitum in yolk sac (YS) Sp. Comparison of Sp and YS precursors from one embryo shows larger differentiation ability and cultural survival for Sp precursors. YS has no capacity for a lymphoid progeny and has insufficient precursors for mixed myeloid colonies.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Definitive hematopoiesis is autonomously initiated by the AGM region
Article Abstract:
A new in vitro organ culture system is developed to investigate definitive adult hematopoiesis in the mouse embryo. Findings of the 9, 10 and 11-day postcoitum (dpc) yolk sac (YS), aorta-agonad-mesonephros (AGM) and liver cultures reveal an independent colony-forming unit-spleen progenitor activity in 10-dpc AGM culture. On the other hand, weak CFU-S activity is observed in YS. A 10-dpc AGM demonstrates active long-term repopulating hematopoietic stem cells activity. Further results implicate AGM as the initiator of the definitive adult hematopoietic system.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton
Article Abstract:
The function of the red blood cell anion exchanger, AE1, in in vivo RBC development is investigated by gene targeting. Specifically, null mutation in mice is carried out by targeted gene disruption in embryonic stem cells. The hematological effects of AE1 deletion do not affect fetal loss of homozygotes. Instances of survival to adulthood and fertility are even reported. Results suggest that AE1 is vital to membrane lipid stability but its plasma membrane localization is not required for either terminal RBC differentiation or membrane skeleton assembly.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Identification of an ER retrieval signal in a retroviral glycoprotein. Point mutations are associated with a gene duplication leading to the bloodstream reexpression of a trypanosome metacylcic VSG
- Abstracts: Spatial autocorrelation analysis of individual multiallele and multilocus genetic structure. Genetic effects of rainforest fragmentation in an early successional tree (Elaeocarpus grandis)
- Abstracts: A method for identification of the expression mode and mapping of QTL underlying embryo-specific characters. Mapping and Exclusion Mapping of Genomic Imprinting Effects in Mouse F2 Families
- Abstracts: Cyanide under Alkaline conditions by a strain of Fusarium solani isolated from contaminated soils. Essential role of cytochrome bd-related oxidase in cyanide resistance of pseudomonas pseudoalcaligenes CECT5244
- Abstracts: The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins. Dissecting RNA-protein interactions: RNA-RNA recognition by Rop