MRC-5 cells induce the AER prior to the duplicated pattern formation in chick limb bud
Article Abstract:
MRC-5 cells, which are human embryonic lung fibroblasts, can induce an apical ectodermal ridge (AER) in the chick limb bud, with excess AER forming a duplicated cartilage pattern. The studies examines the process of duplicated pattern formation by MRC-5 cells grafted in various parts of the limb bud. The duplicated patterns can also be formed after implantation into the center of the limb bud, and an excess AER with Msx2 expression is induced before formation of these duplicated patterns. Alterations in the anteroposterior (A-P) polarity may be due to high polarizing activity of the normal flank region when MRC-5 cells are grafted into the anterior leg bud.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1995
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Digit induction by Hensen's node and notochord involves the expression of shh but not RAR-beta2
Article Abstract:
Hensen's node is a cluster of organizer cells that can induce the formation of supernumerary digits after grafting into the anterior region of developing limb buds. In this study, it was shown that digit induction is correlated with prospective notochord cells, but not prospective somite cells. This inducing ability begins at the early stages of development and persists into the later stages. Probes for retinoic acid receptor-beta2 and sonic hedgehog (shh) showed that digit induction is related to expression of shh.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1996
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Expression of AV-1, a position-sppecific molecule, in response to retinoic acid beads and ZPA grafts in chick limb development
Article Abstract:
An experiment conducted to determine the effect of retinoic acid (RA) and zone polarizing activity (ZPA) grafts revealed that RA is responsible for the transformation of anterior limb bud cells into ZPA cells. Both ZPA and RA produced changes in the expression of AV-1 antigen, a glycoprotein present during the development of the chick limb, but these changes in antigen expression occurred earlier with the ZPA grafts.
Publication Name: Developmental Biology
Subject: Biological sciences
ISSN: 0012-1606
Year: 1993
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