Measurement of antimicrobial-resistant Escherichia coli in pig feces with a hydrophobic grid membrane filter interpreter system
Article Abstract:
A study was conducted to assess the viability of using hydrophobic grid membrane filter (HGMF) on measuring resistance among Escherichia coli in pig fecal samples or any large number of isolates with reasonable laboratory resource demands. The fecal E. coli were tested for resistance to microbials which include ampicillin, sulfisoxazole, and tetracycline. HGMF has been proven to have an advantage over other screening methods such as the GNI system and Cathra Repliscan since it is equipped with the ability to measure multiple resistance attributes of colonies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) prevents proteolytic degradation of ompA by cell-associated protease(s) in two different gram positive bacteria
Article Abstract:
The use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) was examined. An analysis was conducted to prove its effectiveness in preventing proteolytic degradation of OmpA by cell-associated protease in two different gram-positive bacteria. Results show that the use of pre-pro part of a lipase from S. hyicus as secretion vector caused the release of a soluble propeptide-OmpA hybrid protein into the supernatant.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Expression of outer membrane proteins in Escherichia coli growing at acid pH
Article Abstract:
Outer membrane protein C is expressed at low osmolarity in acidic media and is repressed when medium osmolarity is increased. This is the opposite of the effect of osmolarity at neutral and alkaline pH. The expression of outer membrane protein F is similar regardless of the pH of the medium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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