Measurement of growth at very low rates (mu greater than or equal to 0), an approach to study the energy requirement for the survival of Alcaligenes eutrophus JMP 134

Article Abstract:

The flux of substrate which provides the minimum energy required for maintenance allows the growth of Alcaligenes eutrophus JMP 134 at a rate greater than or equal to 0. The microorganism requires 2.4 micromoles of ATP per gram of biomass/hr with fructose as substrate. The ATP requirement with phenol is 1.7 times higher, and six times higher with 2,4-dichlorophenoxyacetic acid (2,4-D). The increased energy expenditure with 2,4-D is probably due to uncoupling effects. The growth patterns are, however, similar with all the substrates and have three phases of linear growth.

Observations, Microbial growth, Adenosine triphosphate, ATP

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Regiospecificity of dioxygenation of di- to pentachlorobiphenyls and their degradation to chlorobenzoates by the bph-encoded catabolic pathway of Burkholderia sp. strain LB400

Article Abstract:

The biphenyl dioxygenase of Burkholderia sp. strain LB400 dioxygenates polychlorobiphenyls at different positions on the ring depending on the placement of substituents. The 2,4-disubstituted ring is dioxygenated at positions 2 and 3, whereas the 2,5-dichlorinated ring is dioxygenated at positions 3 and 4.

Polychlorinated biphenyls

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A theoretical study on the metabolic requirements resulting from alpha-ketoglutarate-dependent cleavage of phenoxyalkanoates

Article Abstract:

Bacteria that cleave phenoxyalkanoates via an alpha-ketoglutarate-dependent dioxygenase would run out of alpha-ketoglutarate and would stop growing. On the other hand, the cleavage of phenoxypropionate and phenoxybutyrate regenerates alpha-ketoglutarate.

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