Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414
Article Abstract:
Genomic and cDNA clones encoding EG III were isolated to characterize and determine the heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. The nucleotide sequence of the cDNA fragment was verified to contain a 702-bp open reading frame that encodes a 234-amino-acid propeptide. The protein sequence was found to contain significant homologies with family H endo-beta-1,4-glucanases. The 16-amino-acid N-terminal sequence was shown to function as a leader peptide for possible secretion.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
An alpha-L-arabinofuranosidase from Trichoderma reesei containing a noncatalytic xylan-binding domain
Article Abstract:
Research was conducted to examine an alpha-L-arabinofuranosidase (alpha-AF) from Trichoderma reesei containing a noncatalytic xylan-binding domain. The alpha-AF was purified and partially characterized and its cDNA was cloned. It was observed in the preliminary results that alpha-AF activity was induced by L-sorbose, an excellent inducer for the production of xylanase and cellulase by T reesei. It is concluded that the enzyme having the lower molecular mass is the truncated form of the enzyme with the higher molecular mass.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Purification and characterization of exo-beta-D-glucosaminidase from a cellulolytic fungus, Trichoderma reesei PC-3-7
Article Abstract:
Tbe identification, purification and characterization of the exo-beta-D-glucosaminidase from the hyper-cellulolytic fungus, Trichoderma cellulolytic fungus, Trichoderma reesei PC-3-7, are described. The exo-beta-D-glucosaminidase was found to possess a retaining catalytic mechanism as it produced a beta-form of glucosamine (GlcN) and to continuously release one GlcN residue from the nonreducing end of the substrate.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Characterization and expression of the hydrogenase-encoding gene from Clostridium acetobutylicum P262. A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis
- Abstracts: Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor. Regulation of laccase gene transcription in Trametes versicolor
- Abstracts: Molecular biology of aging. MEC1-dependent redistribution of the Sir3 silencing protein from telomeres to DNA double-strand breaks
- Abstracts: Characterization of IS2112, a new insertion sequence from Rhodococcus, and its relationship with mobile elements belonging to the IS110 family
- Abstracts: Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)