Molecular characterization of adiY, a regulatory gene which affects expression of the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia coli
Article Abstract:
The Escherichia coli adiY transcriptional regulator gene present downstream of the arginine decarboxylase (adiA) gene encodes the AdiY protein that is similar to the transcriptional regulators of the XylS/AraC family. AdiY contains 253 amino acids and a helix-turn-helix in its C-terminus. Its G+C content is less than the total G+C content of the E. coli genome. AdiY enhances the adiA expression and is expressed during both aerobic and anaerobic conditions. The amino acids necessary for the acid activation of adiA are required for the stimulatory activity of adiY.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-alpha-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli
Article Abstract:
A construction of the gene library in Escherichia coli of the genome of an amylolytic strain of the Clostridium butyricum was conducted to screen for clones capable of degrading amylose. Chromosomal DNA OF C. butyricum was subjected to bacterial strains, plasmids and growth conditions and enzyme assays. Findings of the study showed that no cloned gene contained a common DNA. The smallest fragments that could be cloned further resided between the NspHI and ScaI sites. Furthermore, glucose and maltose were identified to function as acceptor molecules.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Characterization of cell-cycle-specific events in synchronous cultures of Escherichia coli: a theoretical evaluation
Article Abstract:
The development and application of a model of a synchronous culture of Escherichia coli cells to stable and unstable cellular components enable the analysis of the timing, presence and duration of periodicity in bacterial cell cycle. The analysis enables easy identification of the existence of cell cycle-specific events but not of the duration and timing. Interpretations derived using qualitative analysis of the results may misrepresent differences in timing as differences in duration.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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