Molecular detection of Mycobacterium bovis and Mycobacterium bovis BCG (Pasteur) in soil
Article Abstract:
The presence of Mycobacterium bovis BCG (Pasteur) in soil microcosms and Mycobacterium bovis in environmental samples taken from a farm in Ireland with a history of bovine tuberculosis is detected. The results suggest that M. bovis BCG remains viable in soil for more than 15 months and that significant levels of M. bovis DNA and RNA persist in the field, indicating the presence of viable cells as an environmental reservoir for infection, which might pose a risk to cattle.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Microarray-based detection and typing of the rhizobium nodulation gene nodC: Potential of DNA arrays to diagnose biological functions of interest
Article Abstract:
The construction and validation of the nodulation gene nodC microarray to detect nodC sequences in bacteria are described. This method has generated accurate results with genomic DNA from both bacterial cultures and bacteria-infected plant nodules and has proved to be powerful tool for studying the distribution of genes of interest in the environment, as well as for bacteria identification.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Molecular biological detection of anaerobic gut fungi (Neocallimastigales) from landfill sites
Article Abstract:
Oligonucleotide primers were designed for 18S rRNA genes of members of Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The demonstration of occurrence of members of Neocallimastigales outside mammalian gut, and their distribution across landfill samples examined show their active involvement in cellulose degradation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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