Molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding a beta-xylosidase
Article Abstract:
Research was conducted to examine the molecular cloning and transcriptional regulation of the Aspergillus nidulans xlnD gene encoding the 85-kDA beta-xylosidase. The primary structure of the protein had considerable similarity to the primary structures of the Trichoderma reesei and Aspergillus niger beta-xylodases. Western blot analysis revealed elevated levels of production of the enzyme in overexpressing transformants showing that the cloned sequences existing in pXE1 encode the previously characterized 85-kDa beta-xylosidase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi
Article Abstract:
The expression and functional analysis of a hyperglucosylated glocoamylase in Aspergillus awamori var. kawachi, a parental host, were examined. The study introduced a modified glucoamylase gene with an extra Thr- and Ser-rich Gp-I domain into the mutant parental host. Results indicate that modified glaA was successfully expressed and secreted. Furthermore, the modified glaA had higher digestibility of raw corn starch and higher stabilities in response to heat and hydrogen ion concentration.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Molecular cloning and transcriptional analysis of the Aspergillus terreus gla1 gene encoding a glucoamylase
Article Abstract:
Heterologous hybridization facilitates the cloning of the Aspergillus terreus gla1 gene, which encodes a glucoamylase. Transcriptional analysis of the gene reveals the presence of a protein having a C-terminus starch-binding domain and N-terminal catalytic domain. Examination of the influence of carbon sources on gla1 regulation reveals that starch and maltose stimulate the expression of the gene, while glucose represses the expression.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Starvation stress modulates the expression of the Aspergillus nidulans brlA regulatory gene. The TBP gene from Aspergillus nidulans - structure and expression in Saccharomyces cerevisiae
- Abstracts: Structural, functional and mutational analysis of the pfr gene encoding a ferritin from Helicobacter pylori. Identification of a 29 kDa flagellar sheath protein in Helicobacter pylori using a murine monoclonal antibody
- Abstracts: Extragenic suppressors of a dynein mutation that blocks nuclear migration in Aspergillus nidulans. Identification of developmental regulatory genes in Aspergillus nidulans by overexpression
- Abstracts: Multiple trait analysis of genetic mapping for quantitative trait loci. Maximum likelihood mapping of quantitative trait loci using full-sib families
- Abstracts: Expression and in vitro assembly of recombinant glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus