Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by Dehalococcoides-containing cultures

Article Abstract:

Degenerate primers are used to amplify 14 distinct reductive-dehalogenase-homologous (RDH) genes from the Dehalococcoides-containing mixed culture KB1 and also to understand which of these RDH genes have an active role in reductive dechlorination of trichloroethene (TCE), cis-1,2-dichloroethene (cDCE), vinyl chloride (VC) and 1,2-dichloroethane (1,2-DCA). The results suggest that multiple RDH genes are induced by a single chlorinated substrate and that multiple reductive dehalogenases contribute to chloroethene degradation in KB1.

Trichloroethane, Dechlorination, Bacterial genetics, Trichloroethane (1,1,1-trichloroethane)

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A 1,1,1- Trichloroethane-degrading anaerobic mixed microbial culture enhances biotransformation of mixtures of chlorinated ethenes and ethanes

Article Abstract:

MS, a 1,1,1 -Trichloroethane (TCA)-degrading, anaerobic, mixed microbial culture derived from a 1,1,1-TCA- contaminated site in the northeastern United States, is characterized and the capacity of MS to enhance trichloroethene (TCE) degradation in the presence of inhibiting levels of 1,1,1-TCA is tested. The potential application of the MS and MS and KB-1 cultures for cobioaugmentation of sites cocontaminated with 1,1,1-TCA and TCE is demonstrated.

Canada, Drugs, In-Vitro Diagnostic Substance Manufacturing, Bacterial Culture Products, Physiological aspects, Growth, Anaerobic bacteria, Microbial contamination, Bacteriology, Bacterial cultures, Company growth, Nucleotide sequencing

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Characterization of a highly enriched Dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene

Article Abstract:

A highly enriched Dehalococcoides-containing culture that reductively dechlorinates trichloroethene (TCE), cis-1,2-dischloroethene (cDCE), and vinyl chloride (VC) to ethene without methanogenesis is characterized. It was found that the parent TCE-dechlorinating enrichment culture (KB-1) contained two distinct strains of Dehalococcoides, while only one of these strains remained in the VC-H2 enrichment culture.

Vinyl Chloride Monomer, Genetic aspects, Methanobacteriaceae, Methanogens, Microbiological research

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