Two novel bacterial biosensors for detection of nitrate availability in the rhizosphere

Article Abstract:

The development of two whole-cell bacterial reporters to detect the availability of nitrate in soil around roots that is based on Enterobacter cloacae harboring nitrate-responsive reporter genes is reported. The results indicate that both green fluorescent protein (GFP) and inaZ whole-cell biosensors are highly responsive and that the population-level estimates of nitrate availability provided by the ice nucleation-based biosensors are complementary to the estimates of nitrate availability at the single-cell level provided by the GFP-based biosensor.

United States, Usage, Biosensors, Soils, Soil nitrogen

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Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation

Article Abstract:

A high-density polygenitic DNA microarray (16S microarray) containing 500,000 probes is employed to monitor changes in bacterial populations during redemption process to determine the relation between microbial community composition and less-soluble uranium U (IV) reoxidation. Observations suggest that reoxidation of uranium under reducing conditions occur despite elevated microbial activity and consistent presence of metal-reducing bacteria.

Primary nonferrous metals, not elsewhere classified, Primary Smelting and Refining of Nonferrous Metal (except Copper and Aluminum), All Other Basic Inorganic Chemical Manufacturing, Uranium Metal, Industrial inorganic chemicals, not elsewhere classified, Reprocessed Activated Uranium, Environmental aspects, Uranium, DNA microarrays

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Sensitive whole-cell biosensor suitable for detecting a variety of N-acyl homoserin lactones in intact rhizosphere microbial communities

Article Abstract:

A whole-cell biosensor, Agrobacterium tumefaciens is developed for the detection of a variety of N-acyl homoserine lactones (AHLs) in various intact rhizosphere microbial communities. The biosensor is extremely sensitive and shows that the AHL availability shows more increment in such communities, as compared to the bulk soil.

Analysis, Physiological aspects, Lactones, Agrobacterium tumefaciens

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