Mutants of Streptomyces roseosporus that express enhanced recombination within partially homologous genes
Article Abstract:
Homologous recombination in Streptomyces roseosporus mutants was investigated. The bacteriophage phiC31 derivative of KC570 was introduced into a Tn5099-10 transposition mutant library of S. roseosporus and was screened for transductants. Putative ehr mutants showed 10(super 2)- to 10(super 4)-fold increases in homeologous recombination. Southern hybridization analysis showed that the plasmids were inserted into the mutants through single unique sites in the chromosomes. Crossovers during translation resulted in the formation of functional chimeric glnA and whiG genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Gene transfer and transposition mutagenesis in Streptomyces roseosporus: mapping of insertions that influence daptomycin or pigment production
Article Abstract:
Plasmid DNA was introduced into Streptomyces roseosporus by bacteriophage-FP43-mediated transduction and by conjugation from Escherichia coli. Also, the streptomycete transposons Tn5099 and Tn5096 were transposed in S. roseosporus, and several mutants that affect secondary metabolite production were mapped to DraI and AsnI fragments of the chromosome. Three auxotrophic mutations (argB1, ade-1 and metB1) were identified among 100 individual Tn5096 insertions.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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A gene cloning system for 'Streptomyces toyocaensis.'
Article Abstract:
The organization and regulation of glycopeptide biosynthetic genes is studied. The construction of stable recombinant strains was attempted by using various methods to introduce DNA into Streptomyces toyocaensis and Streptomyces virginiae. S. toyocaensis produces the antibiotic A47934, while S. virginiae produces the antibiotic A41030A. Results show that S. toyocaensis functions as an ideal host for gene cloning compared to S. virginiae.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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