Mutational analysis of mesentericin Y105, an anti-Listeria bactriocin, for determination of impact on bactericidal activity, in vitro secondary structure, and membrane interaction
Article Abstract:
The tryptophan fluorescence and circular dichroism (CD) analyses of MesY105 derivatives obtained by randomized mutagenesis or chemical synthesis is presented. The role of specific amino acids in the binding to phospholipid vesicles, in an attempt to determine the structure and mode of action of bacteriocins are investigated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Analysis of the two-peptide bacteriocins lactococcin G and enterocin 1071 by site-directed mutagenesis
Article Abstract:
The analysis of two peptides (Lcn-[alpha] and Lcn-[beta]) of the two-peptide bacteriocin lactococcin G (Lcn) by stepwise site-directed mutagenesis into the corresponding peptides (Ent-[alpha] and Ent-[beta]) of the two-peptide bacteriocin enterocin 1071 is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126
Article Abstract:
Recombinant class IIa bacteriocins are produced by a novel expression system and then purified and applied to piscicolin 126. The methods used and the results produced are discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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