Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms
Article Abstract:
Gene transfer can occur between Acinetobacter spp. and dead bacteria present in soil. This was the conclusion of researchers who analyzed the transfer of a gene for kanamycin resistance from dead bacteria to a kanamycin-sensitive strain of Acinetobacter.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Transformation of Acinetobacter sp. strain BD413(pFG4deltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants
Article Abstract:
Researchers show that a gene for antibiotic resistance can be passed from a transgenic plant to soil bacteria. Plant DNA can persist in soil for long periods of time when plants decay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Natural transformation and availability of transforming DNA to Acinetobacter calcoaceticus in soil microcosms
Article Abstract:
A small microcosm system was developed to assess the impact of various ecological factors on the transformation frequency of Acinetobacter calcoaceticus BD413 with chromosomal DNA in soil. Analysis of the natural transformation of Acinetobacter calcoaceticus in sterile and nonsterile soil systems indicated the inhibitory effects of the soil matrix on the availability of DNA. Furthermore, the growth of the gram-negative microbe in freshly released DNA was a prerequisite to in situ transformation which occurred in the maize rhizosphere.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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