Negative regulation of the Wee1 protein kinase by direct action of the Nim1/Cdr1 mitotic inducer
Article Abstract:
The role of the wee 1 protein kinase n the regulation of entry into mitosis was investigated. The wee 1 protein suppresses entry into mitosis by mediating the tyrosine phosphorylation of cdc2 protein kinase (p34cdc), which triggers mitosis in eukaryotic cells. The study protocol involved the overexpression in bacteria and insect cells of the nim 1 protein, which induces mitosis in yeast cells by downregulating the wee 1 pathway. The results showed that the wee 1 protein is under negative regulation by phosphorylation, and that the nim 1 protein is a wee 1-specific kinase.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1993
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Binding of 14-3-3 proteins and nuclear export control the intracellular localization of the mitotic inducer Cdc25
Article Abstract:
Nuclear export and binding of 14-3-3 proteins regulate intracellular localization of Cdc25, a mitotic inducer. Binding of 14-3-3 proteins near the nuclear localization sequence of Xenopus Cdc25 suppresses mitosis in the frog. Intracellular localization of green fluorescent protein (GFP) -tagged wild-type Cdc25 and of a mutant that is not able to bind 14-3-3 proteins has been studied. Mutagenesis experiments show that Cdc25 has multiple nuclear export sequences and nuclear export control intracellular localization of Cdc25.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1999
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The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts
Article Abstract:
A Xenopus Cdc6 (Xcdc6) homolog is cloned using degenerate polymerase chain reaction primers that amplify a segment of the homolog's complementary DNA. The role of Xcdc6 in DNA replication and cell cycle control in Xenopus egg extracts is characterized by expressing a six histidine-tagged version of Xcdc6 in baculovirus-infected Sf9 insect cells. Results indicate that the homolog is needed to initiate replication in vertebrates and that its function can be regulated by its interaction with the nuclear envelope.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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- Abstracts: Segregation of storage protein mRNAs on the rough endoplasmic reticulum membranes of rice endosperm cells. A second signal recognition event required for translocation into the endoplasmic reticulum
- Abstracts: RNA export. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs