New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library
Article Abstract:
A gene coding for a thermostable esterase was isolated by functional screening of Escherichia coli cells that is transformed with fosmid environmental DNA libraries constructed with metagenomes from thermal environmental samples. The finding of a new thermostable and thermophillic HSL family esterase emphasizes the importance of thermal environmental metagenomic libraries as a source of isolation of novel genes by functional screening in a heterologous E. coli host.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three- dimensional structure
Article Abstract:
The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 is constructed by homology modeling using Archaeoglobus fugidus esterase as reference, and the thermostability-structure relationship is analyzed. The results verify the predicted 3D structure of EstE1 and the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1 is identified.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Transgalactosylation in a water-solvent biphasic reaction system with [beta]-galactosidase displayed on the surfaces of Bacillus subtilis spores
Article Abstract:
[beta]-Galactosidase ([beta]-Gal) displayed on Bacillus subtilis spores are used as a whole-cell immobilized biocatalyst for transgalactosylation in water-solvent biphasic reaction systems. The spore display has offered a new way to construct robust biocatalysts along with a novel basis for phase transfer biocatalytic processes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Ability of thermophilic lactic acid bacteria to produce aroma compounds from amino acids. Identification and functional analysis of the gene encoding methionine-gamma-lyase in brevibacterium linens
- Abstracts: Rhizosphere selection of highly motile phenotypic variants of Pseudomonas fluorescens with enhanced competitive colonization ability
- Abstracts: Nutrient amendments in soil DNA stable isotope probing experiments reduce the observed methanotroph diversity
- Abstracts: Tolerance of Listeria monocytogenes to cell envelope-acting antimicrobial agents is Dependent on SigB. part 2 Fate of the two-component lantibiotic lacticin 3147 in the gastrointestinal tract
- Abstracts: Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase