Novel O-polysaccharide expression, as a lipid A-core-free form, in a lipopolysaccharide-core-defective mutant of Pseudomonas aeruginosa
Article Abstract:
The O-polysaccharide prepared from PML14e was purified and characterized in terms of structure. Its properties were compared with those of the O-polysaccharide from the parent strain PML14 and the mutant strains PML14b and PML14d. The cell surface nature of the strains in focus was studied using monoclonal antibodies against cell-surface antigens. The epitopes exposed on rough strains were found to be primarily hampered by the covalently linked core oligosaccharide instead of the O-polysaccharide chain.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Yersinia pseudotuberculosis and yersinia pestis show increased outer membrane permeability to hydrophobic agents which correlates with lipopolysaccharide acyl-chain fluidity
Article Abstract:
A study was conducted to analyze the ability of Yersinia spp to repel hydrophobic agents and surfactants. Exponential phase cells were resuspended using saline with or without sodium arsenate. Serial dilutions of hydrophogic agents were prepared in 96-well polystyrene plates. Results showed variations between Yersinia spp in their ability to ward off hydrophobic compounds. Findings also indicated that the differences were associated with the degree of acyl-chain fluidity in lipopolysaccharide.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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The influence of A-band and B-band lipopolysaccharide on the surface characteristics and adhesion of Pseudomonas aeruginosa to surfaces
Article Abstract:
The surface hydrophobicity and surface charge of Pseudomonas aeruginosa PAO1 (O5 serotype) and its isogenic lipopolysaccharide (LPS) derivatives that possess A+B-, A-B+ and A-B- LPS are investigated to determine the LPS types' influence on surface characteristics and adhesion. P. aeruginosa can change its overall surface characteristics to influence adhesion and favor survival via phenotypic variation in the relative expression of A- and B-band LPS.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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