A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences
Article Abstract:
A nucleic acid sequence-based amplification system is a sensitive, specific and reliable method for detection of gene mRNA sequences in Listeria monocytogenes strains and isolates in a variety of dairy and egg products. The system detects low numbers of L. monocytogenes cells inoculated into the food samples after a 48 hr enrichment period in modified listeria enrichment broth. The system reliably detects nucleic acids in samples, and gives positive results in the assays of numerous listeria strains and isolates from the food samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Cloning and characterization of a gene encoding a secreted tripeptidyl aminopeptidase from Streptomyces lividans 66
Article Abstract:
The Streptomyces lividans tripeptidyl aminopeptidase (Tap) gene was cloned, allowing DNA cross-hybridization in other Streptomyces strains. Overexpression of the gene revealed a 55,000 molecular weight protein with amino-terminal degradative activity. Lipases, acyl transferases and esterases were found to have significant sequence homology with the Tap region containing the supposed serine residue active site. An amino-terminal signal sequence and a tripeptide which could be cleaved autocatalytically were also identified.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Improved production of mannanase by Streptomyces lividans
Article Abstract:
The substitution of the natural promoter of the beta-mannanase gene by lacp improves mannanase production by Streptomyces lividans. This increases mannanase production 15 times as compared to production by the clone S. lividans IAF36, and 350 times as compared to production by the wild-type strain S. lividans 1326. The enzyme can also be synthesized on mannan-lacking carbon sources, such as xylan and whey, by using lacp in the shuttle vector pIAF199.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Oligonucleotide array for identification and detection of Pythium species. Identification of Bacillus thuringiensis subsp. kurstaki, strain HD1-like bacteria from environmental and human samples after aerial spraying of Victoria, British Columbia, Canada, with Foray 48B
- Abstracts: The unc-8 and sup-40 genes regulate ion channel function in Caenorhabditis elegans motorneurons. Mutations of the clk-1 gene of Caenorhabditis elegans affect developmental and behavioral timing
- Abstracts: Multiple effects of mutation on expression of alternative cell surface protein genes in Tetrahymena thermophila
- Abstracts: Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones
- Abstracts: Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR