Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (Poly-cis-1,4-Isoprene)
Article Abstract:
An extraceelular protein with strong absorption at 406 nm is purified from cell-free culture fluid of latex-grown Xanthomonas sp. strain 35Y. The purified protein degraded both natural rubber latex and chemosynthetic poly(cis-1,4-isoprene) in vitro by oxidative cleavage of the double bonds of poly(cis-1,4-isoprene).
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Physiological and chemical investigations into microbial degradation of synthetic poly(cis-1,4-isoprene)
Article Abstract:
Research demonstrates that synthetic high-molecular weight poly(cis-1,4-isoprene) and natural rubber are degraded by Streptomyces coelicolor 1A and Pseudomonas citronellolis into their low-molecular weight counterparts. Data on the oxidation of poly(cis-1,4-isoprene) to diketones are presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-isoprene) by a dioxygenase mechanism
Article Abstract:
Oxidative cleavage of poly(cis-1,4-isoprene) is investigated by rubber oxygenase RoxA purified from Xanthomonas sp. Based on the labeling results and the presence of two hemes in RoxA, a model of the enzymatic cleavage mechanism of poly(cis-1,4-isoprene) is proposed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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