On-site DNA extraction and real-time PCR for detection of Phytophthora ramorum in the field
Article Abstract:
A method for DNA extraction from symptomatic plant material in the field and the use of a portable real-time PCR platform for accurate on-site detection of Phytophthora romarum within 2 h is described. The DNA extraction method could be completed within 30 min, and unlike many laboratory extraction methods, does not require centrifugation steps, organic solvents, or the use of liquid nitrogen for sample homogenization.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential
Article Abstract:
A destructive pathogenic variant of the plant pathogen Ralstonia solanacearum, which is isolated in Martinique (French West Indies), is examined. The strains belonging to phylotype II/4NPB should be considered a new pathogenic variant within the phylotype II/4 phylogenetic group, which was identified in Martinique in 1998.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk
- Abstracts: Two novel bacterial biosensors for detection of nitrate availability in the rhizosphere. Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation
- Abstracts: A simple and improved correction for population stratification in case-control studies. An evaluation of power and type I error of single-nucleotide polymorphism transmission/disequilibrium-based statistical methods under different family structures, missing parental data, and population stratification
- Abstracts: Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Identification and characterization of conjugative transposons CTn86 and CTn9343 in Bacterroides fragilis strains
- Abstracts: Ralstonia solanacearum pectin methylesterase is required for growth on methylated pectin but not for bacterial wilt virulence