Optimization of bacteriocin release protein (BRP)-mediated protein release by Escherichia coli: random mutagenesis of the pCloDF13-derived BRP gene to uncouple lethality and quasi-lysis from protein release
Article Abstract:
Bacteriocin release protein (BRP) genes were modified to obtain a BRP derivative that is harmful during high-level expression but is useful for the release of unwanted proteins from Escherichia coli. Random mutagenesis using polymerase chain reaction techniques were conducted on the pCloDF13 BRP gene to optimize the release. BRP derivatives did not cause quasi-lysis and had only a small effect on growth. They function in the release of the periplasmic protein beta-lactamase and the periplasmic K88 molecular chaperone FaeE and in the release of bacteriocin cloacin DF13.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Effect of dietary stress on fecal shedding of Escherichia coli O157:H7 in calves
Article Abstract:
The study examined how dietary stress affected fecal shedding of Escherichia coli O157:H7 in three- to four-month-old calves which were experimentally inoculated. Dietary stress was applied by withholding food prior to inoculation in two groups of calves and after inoculation in a third group. No significant increase in fecal shedding of Escherichia coli was observed in the first two groups. The third group was found to be more vulnerable to infection and shed more Escherichia coli than normal-fed calves.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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