Optimization of differential display of prokaryotic mRNA: application to pure culture and soil microcosms
Article Abstract:
Differential mRNA transcription from both pure-culture and soil-derived bacterial RNA was detected by optimizing the differential display technique in eukaryotic gene discovery. Once optimized, a todC1C2 gene fragment from Pseudomonas putida F1 RNA was detected by using an arbitrary primer for the reverse transcriptase step in conjunction with the same arbitrary primer. To validate the results, an arbitrary primer was used to detect recovery a new salicylate-inducible naphthalene dioxygenase in Burkholderia cepacia JS150.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
The AauR-AauS two-component system regulates uptake and metabolism of acidic amino acids in Pseudomonas putida
Article Abstract:
A [sigma.sup.54] dependent two-component system, the AauR-AauS that controls the uptake and metabolism of acidic amino acids in Pseudomonas putida was identified. The results confirmed that the aau system is a major regulator of acidic amino acid uptake and utilization.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Rhodopseudomonas palustris regulons detected by cross-species analysis of alphaproteobacterial genomes
Article Abstract:
A cross-species analysis of transcription regulation in Rhodopseudomonas palustris is described. 4,963 putative transcription factor binding sites, upstream of 2,044 genes and operons are identified using cross-species Gibbs sampling.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Determination of plasmid DNA concentration maintained by nonculturable Escherichia coli in marine microcosms. Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification
- Abstracts: Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr X F- crosses
- Abstracts: Perpetuating the double helix: molecular machines at eukaryotic DNA replication origins. Chromatin remodeling by ATP-dependent molecular machines
- Abstracts: The generation of antibody diversity through somatic hypermutation and class switch recombination. Exploiting quantitative trait loci in gene discovery
- Abstracts: Regulation of functional diversity within the Nedd4 family by accessory and adaptor proteins. Post-translational modifications influence transcription factor activity: A view from the ETS superfamily