Organization of the gene cluster for biosynthesis of penicillin in Penicillium nalgiovense amd antibiotic production in cured dry sausages
Article Abstract:
Penicillin-producing isolates from cured dry sausages have been identified and the presence of a penicillin gene cluster similar to that in Penicillium chrysogenum and Aspergillus nidulans in the isolates has been investigated. The isolates have been classified as members of P. nalgiovense through DNA fingerprinting and their morphological features. A restriction map of the penicillin gene cluster of P. nalviogense was constructed and there was similarity in the biosynthetic genes pcbAb, pcbC and penDE with that in P. chrysogenum and with A. nidulans.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Characterization of the rrnB operon of the plant pathogen Rhodococcus fascians and targeted integrations of exogenous genes at rrn loci
Article Abstract:
The characterization of the rrnB operon of the plant pathogen Rhodococcus fascians is described. rRNA-encoding regions of R fascians D188 were used as target sites for integration and expression of the exogenous gene cmr which confers chloramphenicol resistance. An integrative vector with a 2.5-kb DNA fragment internal to rrnB was derived for targeted integration of exogenous genes at the rrn loci. The transformants had normal growth rates and displayed stability for the integrated marker.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1
Article Abstract:
Researchers have cloned a six-gene region of Corynebacterium glutamicum that contains the genes for the L11 and L1 ribosomal proteins. These proteins activate the RelA factor, which mediates the hyperphosphorylation of GTP when uncharged tRNA accumulate during amino acid starvation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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