P element transposition contributes substantial new variation for a quantitative trait in Drosophila melanogaster
Article Abstract:
A new approach was used to introduce mutations in Drosophila melanogaster through theP-M system of transposition. This system has been shown to be a powerful mutator for many loci, but it suffers from two basic faults. First, experimentsutilizing P-M translocation lack valid controls, and second, crossing P and M strains introduce variations even in the absence of transposition. The new approach utilizes a P stock microinjected with the ry(super 506) M stock. Usingthis 'co-isogenic' P strain, transpostion can be monitored by using the ry(super 506) M strain as control, since no transpostion can occur in this strain.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Efficient and dispersed local P element transposition from Drosophila females
Article Abstract:
Most insertion of the Drosophila P element takes place locally. However, the frequency of local transposition is higher in females than in males and occurs at an orientation similar to the original one. This suggests that elements are transposed with a high frequency within the same complex. Females exhibit not only higher frequency but more diverse local transposition. This indicate that insertion mutation efficiency can be increased significantly by P elements near the target mutation site.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Preferential transposition of Drosophila P elements to nearby chromosomal sites
Article Abstract:
Two schemes using the rosy geneas a marker were utilized in the study of the tranposition of the Drosophila P elements to chromosomal sites near its original position. Mapping of the recovered transposition 0 to 128 kilobase from the starting site reveals that the frequency of a P element inserting at a nearby site is increased 43-67 fold. This transposition mode is probably useful in increasing insertional mutations in predetermined genomic regions.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Are Drosophila SR drive chromosomes always balanced? Quantitative genetics of seminal receptacle length in Drosophila melanogaster
- Abstracts: Assessment of the ratio of pollen to seed flow in a cline for genetic variation in a quantitative trait. Reproductive processes in two oak (Quercus) contact zones with different levels of hybridization
- Abstracts: Genetic and environmental contributions to variation in human tooth size
- Abstracts: Coupling between transcription termination and RNA polymerase inchworming. Isolation and characterization of sigma(sup)70 -retaining transcription elongation complexes from Escherichia coli
- Abstracts: Detection of noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater